| Registro completo |
| Provedor de dados: |
R. Bras. Zootec.
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| País: |
Brazil
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| Título: |
Cloning and sequencing of c-DNA encoding N-methyl-D-aspartate receptor subunit-1 in the hypothalamus of male sheep
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| Autores: |
Ruiz,Ana Zuley
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| Data: |
2014-04-01
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| Ano: |
2014
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| Palavras-chave: |
Cloning
NMDA receptor
Ovis aries
Ram
Sequencing
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| Resumo: |
The objective of the present study was to report the molecular cloning and determination of the sequences N-methyl-D-aspartate subunit-1 receptor (NMDAR-1) in the hypothalamus of sheep. The re-amplified DNA template for NMDA -1 from the hypothalamus of male sheep was cloned using pBluescript-Sk-plasmids (pBSK, 2958 bp). Purified plasmids containing the NMDA receptor c-DNA were sequenced using the dye-terminator chemistry at the University of Nebraska-Lincoln, Nebraska, USA. Results were entered into the National Center for Biotechnology Web site as accession number AY434689. The sequence of Ovis aries (sheep) NMDAR-1 mRNA from hypothalamus of male sheep has 94-97% homology with Homo sapiens, 94-100% homology with Sus scrofa, and 88-90% homology with Rattus norvegicus NMDAR-1 mRNA sequence, and 94-97% homology with Homo sapiens glutamate receptor and transcript variant NR1-2 and NR1-1 mRNA sequence. These results show high evolutionary conservation of NMDA receptor subunit-1 across species.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000400183
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| Editor: |
Sociedade Brasileira de Zootecnia
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| Relação: |
10.1590/S1516-35982014000400004
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| Formato: |
text/html
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| Fonte: |
Revista Brasileira de Zootecnia v.43 n.4 2014
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| Direitos: |
info:eu-repo/semantics/openAccess
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